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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-protected corn
EN
MZIR260
Yes
SYN-ØØ26Ø-3
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Sydney,Person:Syngenta
, AustraliaPhone: +61 1800 067 108,Fax:Email: CSR@syngenta.com,Website: https://www.syngenta.com.au/,Related OrganizationSyngenta Australia ()Private sector (business and industry)Sydney,
, AustraliaPhone: +61 1800 067 108,Fax:Email: CSR@syngenta.com,Website: https://www.syngenta.com.au/,
The maize (Zea mays) was modified to resist the lepidopteran insect pest, fall armyworm (Spodoptera frugiperda). To achieve this resistance, the LM maize expresses a chimeric gene resulting from the combination of sequences from the cry1Gb and cry1Ig genes of Bacillus thuringiensis and expressing an insecticidal protein, eCry1Gb.1Ig, which is a modified Cry delta-endotoxin with enhanced potency against fall armyworm; the protein has a pore-forming mode of action that selectively damages the midgut epithelium line of feeding larvae, resulting in cell lysis and leading to death of the feeding larvae. As a selectable marker against transformants, the LM maize expresses gene phosphomannose isomerase-15 from Escherichia coli K-12 strain which allows the utilization of mannose as a carbon source. Two cassettes were incorporated into the modified maize, one for the expression of the eCry1Gb.1Ig insecticidal protein and another for the expression of the phosphomannose isomerase protein for selection.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Inbred corn line AX5707
EN
pSYN24795
EN
- Agrobacterium-mediated DNA transfer
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1.993 kb
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1.176 kb
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1.035 kb
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0.025 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101941-3 Ubiquitin gene promoter | Saccharum sp. (Sugar cane)Promoter
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-280810-1 eCry1Gb.1Ig | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-110612-2 Ubiquitin gene terminator | Zea mays (Maize, Corn, MAIZE)Terminator
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
Information on the inserted DNA sequence
The transforming plasmid pSYN24795 carried a transfer-DNA (T-DNA) sequence comprising of two Agrobacterium tumefaciens cassettes:
(1) Expression of the eCry1Gb.1Ig synthetic coding sequence is directed by the sugarcane (Saccharum officinarum) ubiquitin-4 promoter SoUbi4-02, which includes its native intron that enhances transcriptional efficiency. The eCry1Gb.1Ig-03 gene encodes a chimeric Cry protein composed of domains from Cry1Gb (I and II) and Cry1Ig (III), designed to target and disrupt midgut epithelial cells of Spodoptera frugiperda larvae. Due to the ubiquitin promoter’s broad and constitutive transcriptional activity, expression of the insecticidal protein occurs in a range of plant tissues. Termination of transcription is directed by the Zea mays ubiquitin terminator sequence ZmUbi361-05.
(2) Expression of the phosphomannose isomerase gene derived from Escherichia coli K-12 strain is driven by the Zea mays ubiquitin-1 promoter ZmUbi1-43, which also contains a regulatory intron that enhances transcriptional activity. The phosphomannose isomerase sequence encodes an enzyme that enables transformed plant cells to utilize mannose as a carbon source, thereby permitting selection during tissue culture. Because the ubiquitin-1 promoter is also constitutive, allowing for effective selection without conferring herbicide resistance. Transcriptional termination is mediated by the Zea mays ubiquitin-1 terminator Zmubi1-04.
Note:
The transforming plasmid pSYN24795 carried a transfer-DNA (T-DNA) sequence comprising of two Agrobacterium tumefaciens cassettes:
(1) Expression of the eCry1Gb.1Ig synthetic coding sequence is directed by the sugarcane (Saccharum officinarum) ubiquitin-4 promoter SoUbi4-02, which includes its native intron that enhances transcriptional efficiency. The eCry1Gb.1Ig-03 gene encodes a chimeric Cry protein composed of domains from Cry1Gb (I and II) and Cry1Ig (III), designed to target and disrupt midgut epithelial cells of Spodoptera frugiperda larvae. Due to the ubiquitin promoter’s broad and constitutive transcriptional activity, expression of the insecticidal protein occurs in a range of plant tissues. Termination of transcription is directed by the Zea mays ubiquitin terminator sequence ZmUbi361-05.
(2) Expression of the phosphomannose isomerase gene derived from Escherichia coli K-12 strain is driven by the Zea mays ubiquitin-1 promoter ZmUbi1-43, which also contains a regulatory intron that enhances transcriptional activity. The phosphomannose isomerase sequence encodes an enzyme that enables transformed plant cells to utilize mannose as a carbon source, thereby permitting selection during tissue culture. Because the ubiquitin-1 promoter is also constitutive, allowing for effective selection without conferring herbicide resistance. Transcriptional termination is mediated by the Zea mays ubiquitin-1 terminator Zmubi1-04.
Note:
- New Generation Sequencing confirmed absence of plasmid pSYN24795 backbone sequences and the single T-DNA insertion at one locus containing both the eCry1Gb.1Ig and pmi expression cassettes with the expected arrangement;
- Real-time quantitative PCR and Pearson’s chi-square (χ2) analysis were conducted and verified the stable inheritance of the inserted DNA across multiple generations;
- The eCry1Gb.1Ig and phosphomannose isomerase proteins in plant tissue were confirmed using immunoassays and functional enzyme/toxin activity assays, and matched the expected protein identities.
EN
- Food
EN
A single base pair change within the intron of the sugarcane-derived ubiquitin promoter (from Guanine to Thymine at position 1786) was found in the MZIR260 insert.
- EUginius: MZIR260 maize detailed information (SYN-ØØ26Ø-3-7) [URL] [ English ]
- OECD BioTrack Product Database: SYN-ØØ26Ø-3-7 [URL] [ English ]
- FSANZ: A1302 Safety assessment - Food derived from insect-protected corn line MZIR260 [PDF] ( Food Standards Australia New Zealand ) [ English ]
- FSANZ: A1302 - Food derived from insect-protected corn line MZIR260 [URL] [ English ]
- ISAAA GM approval database: Crop event MZIR260 (International Service for the Acquisition of Agri-biotech Applications) [URL] [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
